Magic Red

Magic Red 8,9/10 7782 votes
Product CodeApplicationsPack SizeList PriceQuantity
ICT935
Datasheet
IF 25 Tests
ICT936
Datasheet
IF 100 Tests

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Reagents in the Kit
Pack Size: 25 Tests
Magic Red Substrate MR-(DEVD)2, 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL
Magic Red Substrate MR-(DEVD)2, 100 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL
Test Principle
Caspase Magic Red kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red reagent contains a caspase inhibitor sequence (DEVD)2 linked to a red (Cresyl Violet) fluorescent probe.
The Caspase Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

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Storage
Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT935.pdf
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Magic Red™ Caspase-3/7 KitICT936IF 100 Tests

Product Specific References

References for Caspase-3/7

  1. Wei, Z. et al. (2011) Notch activation enhances the microglia-mediated inflammatory response associated with focal cerebral ischemia.
    Stroke. 42 (9): 2589-94.
  2. Shibata, Y. et al. (2016) Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
    Applied Spectroscopy Reviews. Mar 13 [Epub ahead of print]

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

alamarBlue articles and technical help

Protocols

alamarBlue calculators

Downloads


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PlayerWinGameDate
Christin S.€351,610Redeem The DreamApr 27, 2018
Steen D.€178,800StarburstAug 17, 2017
Gonaseelan P.€175,520Mighty SphinxMar 16, 2020
Terence M.£152,100Redeem The DreamJun 27, 2019
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Belinda B.kr1,014,000Fruity FriendsMay 12, 2020
Denise D.£92,550Mighty SphinxMar 11, 2018
Julius H.€105,900Mighty SphinxSep 15, 2018
Terence M.£87,900Redeem The DreamJul 04, 2019
Maik K.€100,280Fruity FriendsSep 30, 2018
Denise D.£85,250Mighty SphinxMar 12, 2018
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PlayerWinGameDate
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Youssef Z.€6,695.00Hammer of FortuneMar 06, 2021
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Ida H.€3,040.00Queen of DiamondsMar 05, 2021
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WINNER STATS:

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